Nobiletin: efficient and large quantity isolation from orange peel extract
Identifieur interne : 002586 ( Main/Exploration ); précédent : 002585; suivant : 002587Nobiletin: efficient and large quantity isolation from orange peel extract
Auteurs : Shiming Li [États-Unis] ; Haiqing Yu [États-Unis] ; Chi-Tang Ho [États-Unis]Source :
- Biomedical Chromatography [ 0269-3879 ] ; 2006-01.
English descriptors
- KwdEn :
- Chromatography, High Pressure Liquid (methods), Citrus sinensis (chemistry), Flavones (isolation & purification), Magnetic Resonance Spectroscopy, Plant Extracts (chemistry), chiral Whelk‐O column, large amount isolation, nobiletin, polymethoxyflavones, silica gel chromatography, sweet orange peel extract.
- MESH :
- chemical , chemistry : Plant Extracts.
- chemical , isolation & purification : Flavones.
- chemistry : Citrus sinensis.
- methods : Chromatography, High Pressure Liquid.
- Magnetic Resonance Spectroscopy.
Abstract
It is known that nobiletin possesses anticancer, antiviral and anti‐inflammatory activities. Recently, the demand for nobiletin in large quantities and high purity has increased. However, conventional normal‐phase silica gel chromatography and C18‐reverse‐phase separation methods cannot satisfy the requirement of pure and gram scale nobiletin in a timely manner. In exploring the composition and the biological activities of polymethoxyflavones from sweet orange (Citrus sinensis) peel, we developed an efficient isolation method for nobiletin. By employing this methodology, pure nobiletin, in gram quantities, was obtained in only one purification cycle. The orange peel extract was loaded onto a silica gel flash column and eluted by a mixed solvent system of ethyl acetate and hexanes, and the fractions collected. Upon combination of the eluted fractions, mainly containing nobiletin and 5,6,7,4′‐tetramethoxyflavone, and concentration under reduced pressure, the resultant residue was loaded onto a Regis chiral column. Gram amounts of nobiletin and 5,6,7,4′‐tetramethoxyflavone were then eluted with ethanol and hexanes, respectively. Copyright © 2005 John Wiley & Sons, Ltd.
Url:
DOI: 10.1002/bmc.540
Affiliations:
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Chromatogr.</title><idno type="ISSN">0269-3879</idno><idno type="eISSN">1099-0801</idno><imprint><publisher>John Wiley & Sons, Ltd.</publisher><pubPlace>Chichester, UK</pubPlace><date type="published" when="2006-01">2006-01</date><biblScope unit="volume">20</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="133">133</biblScope><biblScope unit="page" to="138">138</biblScope></imprint><idno type="ISSN">0269-3879</idno></series><idno type="istex">3C3F69749C33D22B040FDEB2B871A9CF162CE57D</idno><idno type="DOI">10.1002/bmc.540</idno><idno type="ArticleID">BMC540</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0269-3879</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chromatography, High Pressure Liquid (methods)</term><term>Citrus sinensis (chemistry)</term><term>Flavones (isolation & purification)</term><term>Magnetic Resonance Spectroscopy</term><term>Plant Extracts (chemistry)</term><term>chiral Whelk‐O column</term><term>large amount isolation</term><term>nobiletin</term><term>polymethoxyflavones</term><term>silica gel chromatography</term><term>sweet orange peel extract</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Plant Extracts</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Flavones</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus sinensis</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, High Pressure Liquid</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Magnetic Resonance Spectroscopy</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">It is known that nobiletin possesses anticancer, antiviral and anti‐inflammatory activities. Recently, the demand for nobiletin in large quantities and high purity has increased. However, conventional normal‐phase silica gel chromatography and C18‐reverse‐phase separation methods cannot satisfy the requirement of pure and gram scale nobiletin in a timely manner. In exploring the composition and the biological activities of polymethoxyflavones from sweet orange (Citrus sinensis) peel, we developed an efficient isolation method for nobiletin. By employing this methodology, pure nobiletin, in gram quantities, was obtained in only one purification cycle. The orange peel extract was loaded onto a silica gel flash column and eluted by a mixed solvent system of ethyl acetate and hexanes, and the fractions collected. Upon combination of the eluted fractions, mainly containing nobiletin and 5,6,7,4′‐tetramethoxyflavone, and concentration under reduced pressure, the resultant residue was loaded onto a Regis chiral column. Gram amounts of nobiletin and 5,6,7,4′‐tetramethoxyflavone were then eluted with ethanol and hexanes, respectively. Copyright © 2005 John Wiley & Sons, Ltd.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>New Jersey</li></region><settlement><li>New Brunswick (New Jersey)</li></settlement><orgName><li>Université Rutgers</li></orgName></list><tree><country name="États-Unis"><region name="New Jersey"><name sortKey="Li, Shiming" sort="Li, Shiming" uniqKey="Li S" first="Shiming" last="Li">Shiming Li</name></region><name sortKey="Ho, Chi Ang" sort="Ho, Chi Ang" uniqKey="Ho C" first="Chi-Tang" last="Ho">Chi-Tang Ho</name><name sortKey="Yu, Haiqing" sort="Yu, Haiqing" uniqKey="Yu H" first="Haiqing" last="Yu">Haiqing Yu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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